The annual honor was bestowed upon Pitch Perfect 2's Rebel Wilson and Adam DeVine during the show.
Recreating their sloppy big screen kiss onstage Saturday, the duo kept it romantic while "We Belong" by Pat Benatar played – despite at first insisting they had "zero" chemistry.
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The onscreen couple follow in the famous footsteps of Rachel McAdams and Ryan Gosling, who won for The Notebook in 2005, and Carmen Electra and Paris Hilton, who recreated their Starchy & Hutch kiss for the audience the year prior.
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Other best kiss titleholders include Silver Linings Playbook costars Jennifer Lawrence and Bradley Cooper, and consecutive four-time winners Kristen Stewart and Robert Pattinson
The 2016 MTV Movie Awards air on Sunday (at 8 p.m. ET) on MTV.
Check out these 5 tips for getting lips worthy of the MTV Movie Awards' best kiss.