During the Manila, Philippines, stop of her Rebel Heart tour, the singer called out an unidentified former employee who turned out to be less than loyal.
"Once I had a Filipino trainer, this beautiful, beautiful girl," she told the crowd. "She was gorgeous and very talented as a trainer. But she f---ed my boyfriend, so I fired her."
"In this case, it was not a happy ending," Madge, 57, continued, repeating the phrase until it received a sufficient amount of laughter from the audience. "I know I sound like I'm cynical and I don't believe in romance and that I think everybody is backstabbing."
Madonna's Son: He Should Have Been Home By Now, Says Source
Madonna is currently embroiled in a custody battle with ex-husband Guy Ritchie over 15-year-old son Rocco, who allegedly left the Rebel Heart tour in December to live with Ritchie in England.
A New York judge reportedly ordered Rocco to return to his mother in the U.S. in December. Madonna visited Rocco in London soon after in an unsuccessful attempt to bring him home.
Madonna and Ritchie, 47, are due in New York Supreme Court for a hearing on March 2.