The cast introduced a never-before-seen clip from the upcoming August anti-hero movie Suicide Squad during the star-studded Los Angeles ceremony.
WATCH: EW News Flash: Suicide Squad director comments on reshoot rumors
After a brief monologue, Will Smith introduced the video with a question: "Are you ready for the worst heroes ever?"
The action-packed clip featured a hail of gunfire, witty one-liners and Jared Leto sporting The Joker's quintessential makeup.
The movie – which stars Smith, Leto and Margot Robbie – brings together a motley crew of supervillains, united to aide in a secret government mission.
Directed by David Ayer and produced by Zack Snyder, Suicide Squad features many iconic comic book characters, including Harley Quinn (Robbie), the Joker (Leto) and Deadshot (Smith).
Jared Leto: 44 Years, 24 Hairstyles
Smith was also honored with the MTV Generation Award at the show.