02/20/2006 AT 09:00 AM EST
Barry and Robin Gibb, two-thirds of the Bee Gees, reunited on stage for a Miami charity concert on Saturday, their first performance since the death of brother Maurice three years ago.
The private concert at the Diplomat Hotel raised money for the Diabetes Research Institute at an annual benefit for the organization, said Barry Gibb's publicist, Paul Bloch.
Among the dozens of Bee Gees hits they performed from 35 years' worth of songs were "Stayin' Alive," "Massachusetts," "How Deep is Your Love" and a tribute to Maurice, "Don't Forget to Remember."
Maurice Gibb, then 53, died in January 2003 after emergency surgery for an intestinal blockage. He had been admitted to a Miami hospital four days earlier and had suffered cardiac arrest before the operation.
Younger Gibb brother, Andy – who had a successful solo career – died in 1988 at age 30 of a heart ailment.
With nine No. 1 songs and more than 110 million records sold, the Bee Gees rank fifth in pop standings, behind Elvis Presley, the Beatles, Michael Jackson and Paul McCartney.