Despite working through a separation last year, the Medium actress and Hung actor have decided to get a divorce, his rep tells PEOPLE.
"The split is completely amicable and their prime focus is their daughter," says the rep. "They ask that their privacy be respected at this time."
The actress first filed for divorce from Jane in January 2009, but the two reconciled later that year.
Arquette, 42, and Jane, 41, met through friends in 2001 and were married in Italy in May 2006. Their only child together, a daughter named Harlow Olivia Calliope, was born in February 2003.