The Medium star filed for divorce from the actor last Friday, Los Angeles court records show. Arquette is seeking primary physical and legal custody of their daughter Harlow, 7, and is asking the court to deny Jane any spousal support.
"The split is completely amicable and their prime focus is their daughter," a rep for Jane said last week. "They ask that their privacy be respected at this time."
The actress first filed for divorce from Jane, who stars in HBO's Hung, in January 2009, but the two reconciled later that year.
Arquette, 42, and Jane, 41, met through friends in 2001 and were married in Italy in May 2006.