The Desperate Housewives star's documents were filed in Los Angeles Superior Court just one day after her rep denied that Parker had done the same in a Texas court.
The petition cites only the standard "irreconcilable differences" for the split. But this past week the couple have been rocked with infidelity rumors, with claims that Tony has been unfaithful.
Longoria is seeking spousal support from Parker. The couple have a prenuptial agreement signed the month before their wedding and updated two years later. The petition says their date of separation is still to be determined.
Longoria's attorney, Lance Spiegel, wasn't immediately available for comment.
Elaborate WeddingLongoria, 35, and Parker, 28, were engaged in November 2006, then wed – as fireworks lit up the sky – on July 7, 2007, at Chateau of Vaux le Vicomte, a 17th Century castle on the outskirts of Paris one day after a small civil service. The double wedding celebrating came after a week of partying in Saint-Tropez, France.
In 2008, one year after their wedding, the Desperate Housewives star was plagued by pregnancy rumors, though she, Parker and her costars repeatedly denied them.
Still, Longoria Parker, said she was looking forward to having children with her husband, telling reporters in July 2008 that was eager to start a family. "I can't wait," she said at the time.
A year earlier, the two also faced rumors that the San Antonio Spurs star had cheated on his wife, though Longoria Parker stood by her husband, cheering him on at games and sharing public dinners.
"They are perfectly happy, despite everything that's going on," a source told PEOPLE at the time.
And, recently, the actress has pampered her husband, showering him with gifts for his 28th birthday this may. They also celebrated their third anniversary in lavish (and romantic) style with an Adriatic cruise and vacation with friends.