The R&B star, who had a role in Stomp the Yard 2, will appear on an episode of CSI: NY, a rep at the CBS show confirms exclusively to PEOPLE.
"Ne-Yo will be playing a professional hit man who doesn't fit the usual image of a hired killer," the rep says. "This guy carries himself with elegant style but a violent efficiency."
The episode is scheduled to air in February 2011 and there are plans to feature music from Ne-Yo's latest album, Libra Scale.
CSI: NY, which has hosted Kim Kardashian in a past episode, airs Fridays (9 p.m. ET) on CBS.