The Dexter stars, who play brother and sister on the Showtime series, are ending their two-year marriage, their reps tell Entertainment Weekly.
"Having been separated for some time, Jennifer Carpenter and Michael C. Hall have filed for divorce," the reps say in a joint statement.
The announcement comes just one day after Carpenter attended Dexter's fifth season finale party without Hall, Access Hollywood reports.
Carpenter, 31, famously stood by Hall's side during his battle with Hodgkin's lymphoma.
They wed on New Years Eve in 2009. Carpenter gushed to PEOPLE in the past that Hall, 39, is "the sexiest man alive. He's one of the best we've got. He's an incredible talent and if anyone deserves accolades, it's him."