"Though we have weathered many storms together, we recently made the tough decision to engage in a trial separation," they tell PEOPLE exclusively.
"Matt remains living on the farm in our guest house and we work together everyday on the farm, on our business endeavors and most importantly, raising our amazing children."
The Roloffs and their four children – twins Jeremy and Zack, 23, Molly, 20, and Jacob, 17 – starred in the reality series Little People, Big World, which chronicled the daily lives of the six-member family, three of whom were diagnosed with dwarfism.
"Last year it was long and tough," Matt says in a clip for a new special with the family airing March 25 at 9 p.m. on TLC.
"Amy and I had a lot of tension, so right around Thanksgiving, I thought I would move over to the bridal house for a little bit and we'll try that. Amy and I stuck it out for years and years when maybe we shouldn't have. I never quite felt at home in my own home so something needed to change," he added. "We needed to try something different."
"I think what Matt and I failed to do over many, many years is learned to live together," says Amy. "It's not my choice for him to leave. I am saddened by it. We don't have a plan as far as how we are going to work this out, so it's not going to be easy."
While the separated pair continue to focus on their children during this difficult time, they tell PEOPLE they are grateful for the support from those around them.
"We sincerely appreciate the unwavering support, understanding and prayers from our family, friends and many fans. May God bless you."