"Where are the English subtitles? We have no idea what @AmericaFerrera and @EvaLongoria are saying," the news and entertainment channel wrote in a since-deleted tweet.
Eva Longoria: My Dog Is Definitely a Desperate Housewife
Before presenting the award for best actor in a television series, drama, American-born Latinas Ferrera, 31, and Longoria, 40, joked about being confused with other Hispanic actresses.
"Hi, I'm Eva Longoria, not Eva Mendes," said Longoria.
"And hi, I'm America Ferrera, not Gina Rodriguez," said Ferrera, who was previously misidentified as the Jane the Virgin star on the official Golden Globes Twitter account.
After receiving major backlash for the tweet, MTV Australia deleted it and attempted to make amends.
"Our Tweet was in reference to @EvaLongoria & @AmericaFerrera's #GoldenGlobes joke. We sincerely apologise for causing offence," they wrote.
We get it was a bad call. We’ll leave the humour to @rickygervais— MTV AUSTRALIA (@MTVAUSTRALIA) January 11, 2016
Shortly after, they added, "We get it was a bad call. We'll leave the humour to @rickygervais."